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superscript iii reverse transcriptase first strand cdna synthesis kit  (Thermo Fisher)


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    Thermo Fisher superscript iii reverse transcriptase first strand cdna synthesis kit
    Superscript Iii Reverse Transcriptase First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+iii+reverse+transcriptase+first+strand+cdna+synthesis+kit/RevertAid+Reverse+Transcriptase+(200+U%2F%C2%B5L)/pm36706536-87-10-19
    Average 90 stars, based on 1 article reviews
    superscript iii reverse transcriptase first strand cdna synthesis kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Comparison of Surrogate Markers of the Type I Interferon Response and Their Ability to Mirror Disease Activity in Systemic Lupus Erythematosus
    Article Snippet: .. Total RNA extraction from PBMCs was performed using the ISOLATE II RNA Mini Kit from Bioline (London, UK) and cDNA was synthesised by using the SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, CA, USA). .. For quantitation of gene transcripts, the SensiFAST SYBR ® Hi-ROX Kit (Bioline) was used and analysed in a CFX96 Touch Real-Time system (BIO-RAD Inc.).

    Article Title: Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells
    Article Snippet: .. 185 186 Total RNA extraction, reverse transcription and qPCR 187 Total RNA from DCs exposed to mock, F-HSV2, C-HSV2, C1-HSV2, and C2-HSV2 was 188 extracted using Isolate II RNA Mini Kit (Bioline, UK) and total cDNA was produced by 189 SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, 190 CA, USA). .. Quantification of gene transcripts was performed using the SensiFAST SYBR® Hi-191 ROX Kit (Bioline, UK) using a 7900HT Fast Real Time PCR system with 7900 System SDS 2.3 192 Software (Applied Biosystems, Sweden).

    Reverse Transcription:

    Article Title: Comparison of Surrogate Markers of the Type I Interferon Response and Their Ability to Mirror Disease Activity in Systemic Lupus Erythematosus
    Article Snippet: .. Total RNA extraction from PBMCs was performed using the ISOLATE II RNA Mini Kit from Bioline (London, UK) and cDNA was synthesised by using the SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, CA, USA). .. For quantitation of gene transcripts, the SensiFAST SYBR ® Hi-ROX Kit (Bioline) was used and analysed in a CFX96 Touch Real-Time system (BIO-RAD Inc.).

    Article Title: Metabolic adaptations associated with irreversible glucose loss are different to those observed during under-nutrition.
    Article Snippet: In this study the hypothesis that irreversible glucose loss results in an ‘uncoupling’ of the somatotrophic axis (increasing plasma H levels and decreasing plasma IGF-I) was tested.. During periods of negative energy balance the somatotrophic axis respond y increasing plasma GH and decreasing plasma IGF-I levels.. In turn, elevated GH repartitions nutrient by increasing lipolysis nd protein synthesis, and decreases protein degradation.

    Article Title: Conformational state of C-reactive protein is critical for reducing immune complex-triggered type I interferon response: Implications for pathogenic mechanisms in autoimmune diseases imprinted by type I interferon gene dysregulation.
    Article Snippet: Total RNA was extracted from PBMCs using the Isolate II RNA Mini Kit (Bioline, UK). .. Conversion into complementary DNA (cDNA) was performed by using the SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, CA, USA). .. Measurement of mRNA expression of IFNα4, IFNα10, IFNβ, TNF, and transforming growth factor-β (TGFβ) (for primer sequences, see Supplementary Table 3) with quantitative PCR was achieved by using SensiFAST SYBR® Hi-ROX Kit (Bioline, UK) and the CFX96 Touch RealTime system (BIO-RAD Inc.).

    Article Title: Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells
    Article Snippet: .. 185 186 Total RNA extraction, reverse transcription and qPCR 187 Total RNA from DCs exposed to mock, F-HSV2, C-HSV2, C1-HSV2, and C2-HSV2 was 188 extracted using Isolate II RNA Mini Kit (Bioline, UK) and total cDNA was produced by 189 SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, 190 CA, USA). .. Quantification of gene transcripts was performed using the SensiFAST SYBR® Hi-191 ROX Kit (Bioline, UK) using a 7900HT Fast Real Time PCR system with 7900 System SDS 2.3 192 Software (Applied Biosystems, Sweden).

    Article Title: HSV-2 Cellular Programming Enables Productive HIV Infection in Dendritic Cells.
    Article Snippet: .. Total RNA from DCs exposed to the different conditions was extracted using a commercial Isolate II RNA Mini or Micro Kit (Bioline, UK), and total cDNA was produced by SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, CA, USA). .. Quantification of gene transcripts was performed using the SensiFAST SYBR R© Hi-ROX Kit (Bioline, UK) and CFX96 Touch Real-Time system (BIO-RAD Inc.).

    Article Title: Regulation of a quorum sensing system by stationary phase sigma factor RpoS and their co-regulation of target genes in Burkholderia pseudomallei.
    Article Snippet: Each RNA sample was treated with RQ1 RNase-free DNase (Promega, Madison, WI, USA) and tested for DNA contamination. .. Measurement of RNA concentration was performed using Nanodrop 2000 (Thermo Fisher Scientific, Houston, TX, USA). cDNA synthesis was performed using a Superscript III Reverse Transcriptase First Strand cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions. .. Relative quantification real-time PCR for the genes listed in Table 1 was performed using a Rotor-Gene 3000 (Qiagen, Doncaster, Vic, Australia).

    cDNA Synthesis:

    Article Title: Comparison of Surrogate Markers of the Type I Interferon Response and Their Ability to Mirror Disease Activity in Systemic Lupus Erythematosus
    Article Snippet: .. Total RNA extraction from PBMCs was performed using the ISOLATE II RNA Mini Kit from Bioline (London, UK) and cDNA was synthesised by using the SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, CA, USA). .. For quantitation of gene transcripts, the SensiFAST SYBR ® Hi-ROX Kit (Bioline) was used and analysed in a CFX96 Touch Real-Time system (BIO-RAD Inc.).

    Article Title: Metabolic adaptations associated with irreversible glucose loss are different to those observed during under-nutrition.
    Article Snippet: In this study the hypothesis that irreversible glucose loss results in an ‘uncoupling’ of the somatotrophic axis (increasing plasma H levels and decreasing plasma IGF-I) was tested.. During periods of negative energy balance the somatotrophic axis respond y increasing plasma GH and decreasing plasma IGF-I levels.. In turn, elevated GH repartitions nutrient by increasing lipolysis nd protein synthesis, and decreases protein degradation.

    Article Title: Conformational state of C-reactive protein is critical for reducing immune complex-triggered type I interferon response: Implications for pathogenic mechanisms in autoimmune diseases imprinted by type I interferon gene dysregulation.
    Article Snippet: Total RNA was extracted from PBMCs using the Isolate II RNA Mini Kit (Bioline, UK). .. Conversion into complementary DNA (cDNA) was performed by using the SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, CA, USA). .. Measurement of mRNA expression of IFNα4, IFNα10, IFNβ, TNF, and transforming growth factor-β (TGFβ) (for primer sequences, see Supplementary Table 3) with quantitative PCR was achieved by using SensiFAST SYBR® Hi-ROX Kit (Bioline, UK) and the CFX96 Touch RealTime system (BIO-RAD Inc.).

    Article Title: Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells
    Article Snippet: .. 185 186 Total RNA extraction, reverse transcription and qPCR 187 Total RNA from DCs exposed to mock, F-HSV2, C-HSV2, C1-HSV2, and C2-HSV2 was 188 extracted using Isolate II RNA Mini Kit (Bioline, UK) and total cDNA was produced by 189 SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, 190 CA, USA). .. Quantification of gene transcripts was performed using the SensiFAST SYBR® Hi-191 ROX Kit (Bioline, UK) using a 7900HT Fast Real Time PCR system with 7900 System SDS 2.3 192 Software (Applied Biosystems, Sweden).

    Article Title: HSV-2 Cellular Programming Enables Productive HIV Infection in Dendritic Cells.
    Article Snippet: .. Total RNA from DCs exposed to the different conditions was extracted using a commercial Isolate II RNA Mini or Micro Kit (Bioline, UK), and total cDNA was produced by SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, CA, USA). .. Quantification of gene transcripts was performed using the SensiFAST SYBR R© Hi-ROX Kit (Bioline, UK) and CFX96 Touch Real-Time system (BIO-RAD Inc.).

    Article Title: Regulation of a quorum sensing system by stationary phase sigma factor RpoS and their co-regulation of target genes in Burkholderia pseudomallei.
    Article Snippet: Each RNA sample was treated with RQ1 RNase-free DNase (Promega, Madison, WI, USA) and tested for DNA contamination. .. Measurement of RNA concentration was performed using Nanodrop 2000 (Thermo Fisher Scientific, Houston, TX, USA). cDNA synthesis was performed using a Superscript III Reverse Transcriptase First Strand cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions. .. Relative quantification real-time PCR for the genes listed in Table 1 was performed using a Rotor-Gene 3000 (Qiagen, Doncaster, Vic, Australia).

    Real-time Polymerase Chain Reaction:

    Article Title: Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells
    Article Snippet: .. 185 186 Total RNA extraction, reverse transcription and qPCR 187 Total RNA from DCs exposed to mock, F-HSV2, C-HSV2, C1-HSV2, and C2-HSV2 was 188 extracted using Isolate II RNA Mini Kit (Bioline, UK) and total cDNA was produced by 189 SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, 190 CA, USA). .. Quantification of gene transcripts was performed using the SensiFAST SYBR® Hi-191 ROX Kit (Bioline, UK) using a 7900HT Fast Real Time PCR system with 7900 System SDS 2.3 192 Software (Applied Biosystems, Sweden).

    Produced:

    Article Title: Complement Opsonization Promotes Herpes Simplex Virus 2 Infection of Human Dendritic Cells
    Article Snippet: .. 185 186 Total RNA extraction, reverse transcription and qPCR 187 Total RNA from DCs exposed to mock, F-HSV2, C-HSV2, C1-HSV2, and C2-HSV2 was 188 extracted using Isolate II RNA Mini Kit (Bioline, UK) and total cDNA was produced by 189 SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, 190 CA, USA). .. Quantification of gene transcripts was performed using the SensiFAST SYBR® Hi-191 ROX Kit (Bioline, UK) using a 7900HT Fast Real Time PCR system with 7900 System SDS 2.3 192 Software (Applied Biosystems, Sweden).

    Article Title: HSV-2 Cellular Programming Enables Productive HIV Infection in Dendritic Cells.
    Article Snippet: .. Total RNA from DCs exposed to the different conditions was extracted using a commercial Isolate II RNA Mini or Micro Kit (Bioline, UK), and total cDNA was produced by SuperScript III Reverse Transcriptase First Strand cDNA Synthesis kit (Invitrogen, Carlsbad, CA, USA). .. Quantification of gene transcripts was performed using the SensiFAST SYBR R© Hi-ROX Kit (Bioline, UK) and CFX96 Touch Real-Time system (BIO-RAD Inc.).

    Concentration Assay:

    Article Title: Regulation of a quorum sensing system by stationary phase sigma factor RpoS and their co-regulation of target genes in Burkholderia pseudomallei.
    Article Snippet: Each RNA sample was treated with RQ1 RNase-free DNase (Promega, Madison, WI, USA) and tested for DNA contamination. .. Measurement of RNA concentration was performed using Nanodrop 2000 (Thermo Fisher Scientific, Houston, TX, USA). cDNA synthesis was performed using a Superscript III Reverse Transcriptase First Strand cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions. .. Relative quantification real-time PCR for the genes listed in Table 1 was performed using a Rotor-Gene 3000 (Qiagen, Doncaster, Vic, Australia).



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